RNA Interference of Chitinase Gene in Spodoptera litura
نویسندگان
چکیده
منابع مشابه
Down-Regulation of sidB Gene by Use of RNA Interference in Aspergillus nidulans
Background: Introduction of the RNA interference (RNAi) machinery has guided the researchers to discover the function of essential vital or virulence factor genes in the microorganisms such as fungi. In the filamentous fungus Aspergillus nidulans, the gene sidB plays an essential role in septation, conidiation and vegetative hyphal growth. In the present study, we benefited from the RNAi strate...
متن کاملComparison of Amino Acid Sequences of Halloween Genes in Spodoptera litura and Spodoptera littoralis
20-Hydroxyecdyone (20E), an active form of ecdysteroid, is the key hormone in insect growth and development. To date five P450 enzymes involved in the last four steps of ecdysteroid biosynthesis from ketodiol to 20E namely Spook (Spo), Phantom (Phm), Disembodied (Dib), Shadow (Sad) and Shade (Shd) related to ecdysteroid biosynthesis, are identified and the character of last four enzymes is well...
متن کاملIdentification, Expression and Target Gene Analyses of MicroRNAs in Spodoptera litura
MicroRNAs (miRNAs) are small RNAs widely present in animals and plants and involved in post-transcriptional regulation of gene transcripts. In this study we identified and validated 58 miRNAs from an EST dataset of Spodoptera litura based on the computational and experimental analysis of sequence conservation and secondary structure of miRNA by comparing the miRNA sequences in the miRbase. RT-P...
متن کاملEnhancement of RNA Interference Effect in P19 EC Cells by an RNA-dependent RNA Polymerase
Background: RNA interference (RNAi) is a phenomenon uses double-stranded RNA (dsRNA) to specifically inhibit gene expression. The non-specific silencing caused by interferon response to dsRNA in mammalian cells limits the potential of utilizing RNAi to study gene function. Duplexes of 21-nucleotide short interfering dsRNA (siRNA) inhibit gene expression by RNAi. In some organisms, siRNA can als...
متن کاملElongation Factor 1β′ Gene from Spodoptera exigua: Characterization and Function Identification through RNA Interference
Elongation factor (EF) is a key regulation factor for translation in many organisms, including plants, bacteria, fungi, animals and insects. To investigate the nature and function of elongation factor 1β' from Spodoptera exigua (SeEF-1β'), its cDNA was cloned. This contained an open reading frame of 672 nucleotides encoding a protein of 223 amino acids with a predicted molecular weight of 24.04...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: The Korean Journal of Pesticide Science
سال: 2014
ISSN: 1226-6183
DOI: 10.7585/kjps.2014.18.3.202